How fish fins evolved just before the transition to land

Research on fossilized fish from the late Devonian period, roughly 375 million years ago, details the evolution of fins as they began to transition into limbs fit for walking on land.

The new study by paleontologists from the University of Chicago, published this week in the Proceedings of the National Academy of Sciences, uses CT scanning to examine the shape and structure of fin rays while still encased in surrounding rock. The imaging tools allowed the researchers to construct digital 3D models of the entire fin of the fishapod Tiktaalik roseae and its relatives in the fossil record for the first time. They could then use these models to infer how the fins worked and changed as they evolved into limbs.

Much of the research on fins during this key transitional stage focuses on the large, distinct bones and pieces of cartilage that correspond to those of our upper arm, forearm, wrist, and digits. Known as the "endoskeleton," researchers trace how these bones changed to become recognizable arms, legs and fingers in tetrapods, or four-legged creatures.

The delicate rays and spines of a fish's fins form a second, no less important "dermal" skeleton, which was also undergoing evolutionary changes in this period. These pieces are often overlooked because they can fall apart when the animals are fossilized or because they are removed intentionally by fossil preparators to reveal the larger bones of the endoskeleton. Dermal rays form most of the surface area of many fish fins but were completely lost in the earliest creatures with limbs.

"We're trying to understand the general trends and evolution of the dermal skeleton before all those other changes happened and fully-fledged limbs evolved," said Thomas Stewart, PhD, a postdoctoral researcher who led the new study. "If you want to understand how animals were evolving to use their fins in this part of history, this is an important data set."

Seeing ancient fins in 3D

Stewart and his colleagues worked with three late Devonian fishes with primitive features of tetrapods: Sauripterus taylori, Eusthenopteron foordi and Tiktaalik roseae, which was discovered in 2006 by a team led by UChicago paleontologist Neil Shubin, PhD, the senior author of the new study. Sauripterus and Eusthenopteron were believed to have been fully aquatic and used their pectoral fins for swimming, although they may have been able to prop themselves up on the bottom of lakes and streams. Tiktaalik may have been able to support most of its weight with its fins and perhaps even used them to venture out of the water for short trips across shallows and mudflats.

"By seeing the entire fin of Tiktaalik we gain a clearer picture of how it propped itself up and moved about. The fin had a kind of palm that could lie flush against the muddy bottoms of rivers and streams," Shubin said.

Stewart and Shubin worked with undergraduate student Ihna Yoo and Justin Lemberg, PhD, another researcher in Shubin's lab, to scan specimens of these fossils while they were still encased in rock. Using imaging software, they then reconstructed 3D models that allowed them to move, rotate and visualize the dermal skeleton as if it were completely extracted from the surrounding material.

The models showed that the fin rays of these animals were simplified, and the overall size of the fin web was smaller than that of their fishier predecessors. Surprisingly, they also saw that the top and bottom of the fins were becoming asymmetric. Fin rays are actually formed by pairs of bones. In Eusthenopteron, for example, the dorsal, or top, fin ray was slightly larger and longer than the ventral, or bottom one. Tiktaalik's dorsal rays were several times larger than its ventral rays, suggesting that it had muscles that extended on the underside of its fins, like the fleshy base of the palm, to help support its weight.

"This provides further information that allows us to understand how an animal like Tiktaalik was using its fins in this transition," Stewart said. "Animals went from swimming freely and using their fins to control the flow of water around them, to becoming adapted to pushing off against the surface at the bottom of the water."

Stewart and his colleagues also compared the dermal skeletons of living fish like sturgeon and lungfish to understand the patterns they were seeing in the fossils. They saw some of the same asymmetrical differences between the top and bottom of the fins, suggesting that those changes played a larger role in the evolution of fishes.

"That gives us more confidence and another data set to say these patterns are real, widespread and important for fishes, not just in the fossil record as it relates to the fin-to-limb transition, but the function of fins broadly."

Climate signals detected in global weather

In October this year, weather researchers in Utah measured the lowest temperature ever recorded in the month of October in the US (excluding Alaska): -37.1°C. The previous low-temperature record for October was -35°C, and people wondered what had happened to climate change.

Until now, climate researchers have responded that climate is not the same thing as weather. Climate is what we expect in the long term, whereas weather is what we get in the short term -- and since local weather conditions are highly variable, it can be very cold in one location for a short time despite long-term global warming. In short, the variability of local weather masks long-term trends in global climate.

A paradigm shift

Now, however, a group led by ETH professor Reto Knutti has conducted a new analysis of temperature measurements and models. The scientists concluded that the weather-is-not-climate paradigm is no longer applicable in that form. According to the researchers, the climate signal -- that is, the long-term warming trend -- can actually be discerned in daily weather data, such as surface air temperature and humidity, provided that global spatial patterns are taken into account.

In plain English, this means that -- despite global warming -- there may well be a record low temperature in October in the US. If it is simultaneously warmer than average in other regions, however, this deviation is almost completely eliminated. "Uncovering the climate change signal in daily weather conditions calls for a global perspective, not a regional one," says Sebastian Sippel, a postdoc working in Knutti's research group and lead author of a study recently published in Nature Climate Change.

Statistical learning techniques extract climate change signature

In order to detect the climate signal in daily weather records, Sippel and his colleagues used statistical learning techniques to combine simulations with climate models and data from measuring stations. Statistical learning techniques can extract a "fingerprint" of climate change from the combination of temperatures of various regions and the ratio of expected warming and variability. By systematically evaluating the model simulations, they can identify the climate fingerprint in the global measurement data on any single day since spring 2012.

A comparison of the variability of local and global daily mean temperatures shows why the global perspective is important. Whereas locally measured daily mean temperatures can fluctuate widely (even after the seasonal cycle is removed), global daily mean values show a very narrow range.

If the distribution of global daily mean values from 1951 to 1980 are then compared with those from 2009 to 2018, the two distributions (bell curves) barely overlap. The climate signal is thus prominent in the global values but obscured in the local values, since the distribution of daily mean values overlaps quite considerably in the two periods.

Application to the hydrological cycle

The findings could have broad implications for climate science. "Weather at the global level carries important information about climate," says Knutti. "This information could, for example, be used for further studies that quantify changes in the probability of extreme weather events, such as regional cold spells. These studies are based on model calculations, and our approach could then provide a global context of the climate change fingerprint in observations made during regional cold spells of this kind. This gives rise to new opportunities for the communication of regional weather events against the backdrop of global warming."

The study stems from a collaboration between ETH researchers and the Swiss Data Science Center (SDSC), which ETH Zurich operates jointly with its sister university EPFL. "The current study underlines how useful data science methods are in clarifying environmental questions, and the SDSC is of great use in this," says Knutti. Data science methods not only allow researchers to demonstrate the strength of the human "fingerprint," they also show where in the world climate change is particularly clear and recognisable at an early stage. This is very important in the hydrological cycle, where there are very large natural fluctuations from day to day and year to year. "In future, we should therefore be able to pick out human-induced patterns and trends in other more complex measurement parameters, such as precipitation, that are hard to detect using traditional statistics," says the ETH professor.

North Atlantic Current may cease temporarily in the next century

The North Atlantic Current transports warm water from the Gulf of Mexico towards Europe, providing much of north-western Europe with a relatively mild climate. However, scientists suspect that meltwater from Greenland and excessive rainfall could interfere with this ocean current. Simulations by scientists from the University of Groningen and Utrecht University showed that it is unlikely that the current will come to a complete stop, due to small and rapid changes in precipitation over the North Atlantic. However, there is a 15 percent likelihood that there will be a temporary change in the current in the next 100 years. The results were published on 30 December in the journal Scientific Reports.

'The oceans store an immense amount of energy and the ocean currents have a strong effect on the Earth's climate,' says University of Groningen Associate Professor in Numerical Mathematics, Fred Wubs. Together with his colleague Henk Dijkstra from Utrecht University, he has studied ocean currents for some 20 years.

Box model

Ocean scientists have found that the Atlantic Ocean currents are sensitive to the amount of fresh water at the surface. Since the run-off of meltwater from Greenland has increased due to climate change, as has rainfall over the ocean, it has been suggested that this may slow down or even reverse the North Atlantic Current, blocking the transport of heat to Europe.

Simulations of the effects of freshwater on the currents have already been performed for some decades. 'Both high-resolution models, based on the equations describing fluid flows, and highly simplified box models have been used,' explains Wubs. 'Our colleagues in Utrecht created a box model that describes present-day large-scale processes in the ocean rather well.'

Total collapse

The idea was to use this box model to estimate the likelihood of small fluctuations in freshwater input causing a temporary slowing down or a total collapse of the North Atlantic Current. The current shows non-linear behaviour, which means that small changes can have large effects. The evolution of the physics described by the box model can only be obtained using simulations. 'As the transitions we were looking for are expected to be rare events, you need a huge number of simulations to estimate the chance of them happening,' says Wubs. However, the Dutch scientists found that a French scientist had devised a method to select the most promising simulations, reducing the number of full simulations required.

Sven Baars, a PhD student of Wubs, implemented this method efficiently and linked it to the Utrecht box model. Daniele Castellana, a PhD student of Dijkstra, performed the simulations. 'These simulations showed that the chances of a total collapse of the North Atlantic Current within the next thousand years are negligible,' says Wubs.

Interruption

A temporary interruption in the delivery of relatively warm water to north-western Europe is more likely: 'In our simulations, the chances of this happening in the next 100 years are 15 percent.' Such temporary transitions may cause cold spells in the North Atlantic, although this needs to be verified in further studies. Therefore, the current study is just a first step in determining the risk. The model does not take into account considerable changes in freshwater in the North Atlantic, which can be caused by the melting of the ice sheets. Wubs: 'Confirming our results through simulation with a high-resolution climate model will be the next challenge.'

Global extent of river ice loss as Earth warms

More than half of Earth's rivers freeze over every year. These frozen rivers support important transportation networks for communities and industries located at high latitudes. Ice cover also regulates the amount of greenhouse gasses released from rivers into Earth's atmosphere.

A new study from researchers in the University of North Carolina at Chapel Hill Department of Geological Sciences found that annual river ice cover will decline by about six days for every one degree Celsius increase in global temperatures. This decline will have economic and environmental consequences. The study, "The past and future of global river ice," was published Jan. 1 in the journal Nature. It is the first study to look at the future of river ice on a global scale.

"We used more than 400,000 satellite images taken over 34 years to measure which rivers seasonally freeze over worldwide, which is about 56% of all large rivers," said Xiao Yang, a postdoctoral scholar in the UNC-Chapel Hill geological sciences department and lead author on the paper. "We detected widespread declines in monthly river ice coverage. And the predicted trend of future ice loss is likely to lead to economic challenges for people and industries along these rivers, and shifting seasonal patterns in greenhouse gas emissions from the ice-affected rivers."

The team also looked at changes to river ice cover in the past and modeled predicted changes for the future. Comparing river ice cover from 2008-2018 and 1984-1994, the team found a monthly global decline ranging from .3 to 4.3 percentage points. The greatest declines were found in the Tibetan Plateau, eastern Europe and Alaska.

"The observed decline in river ice is likely to continue with predicted global warming," the study explains.

For the future, the team compared expected river ice cover through 2009-2029 and 2080-2100. Findings showed monthly declines in the Northern Hemisphere ranging from 9-15% in the winter months and 12-68% during the spring and fall. The Rocky Mountains, northeastern United States, eastern Europe and Tibetan Plateau are expected to take the heaviest impact.

Researchers learn more about teen-age T.Rex

Without a doubt, Tyrannosaurus rex is the most famous dinosaur in the world. The 40-foot-long predator with bone crushing teeth inside a five-foot long head are the stuff of legend. Now, a look within the bones of two mid-sized, immature T. rex allow scientists to learn about the tyrant king's terrible teens as well.

In the early 2000s, the fossil skeletons of two comparatively small T. rex were collected from Carter County, Montana, by Burpee Museum of Natural History in Rockford, Illinois. Nicknamed "Jane" and "Petey," the tyrannosaurs would have been slightly taller than a draft horse and twice as long.

The team led by Holly Woodward, Ph.D., from Oklahoma State University Center for Health Sciences studied Jane and Petey to better understand T. rex life history.

The study "Growing up Tyrannosaurus rex: histology refutes pygmy 'Nanotyrannus' and supports ontogenetic niche partitioning in juvenile Tyrannosaurus" appears in the peer-reviewed journal Science Advances.

Co-authors include Jack Horner, presidential fellow at Chapman University; Nathan Myhrvold, founder and CEO of Intellectual Ventures; Katie Tremaine, graduate student at Montana State University; Scott Williams, paleontology lab and field specialist at Museum of the Rockies; and Lindsay Zanno, division head of paleontology at the North Carolina Museum of Natural Sciences. Supplemental histological work was conducted at the Diane Gabriel Histology Labs at Museum of the Rockies/Montana State University.

"Historically, many museums would collect the biggest, most impressive fossils of a dinosaur species for display and ignore the others," said Woodward. "The problem is that those smaller fossils may be from younger animals. So, for a long while we've had large gaps in our understanding of how dinosaurs grew up, and T. rex is no exception."

The smaller size of Jane and Petey is what make them so incredibly important. Not only can scientists now study how the bones and proportions changed as T. rex matured, but they can also utilize paleohistology -- the study of fossil bone microstructure -- to learn about juvenile growth rates and ages. Woodward and her team removed thin slices from the leg bones of Jane and Petey and examined them at high magnification.

"To me, it's always amazing to find that if you have something like a huge fossilized dinosaur bone, it's fossilized on the microscopic level as well," Woodward said. "And by comparing these fossilized microstructures to similar features found in modern bone, we know they provide clues to metabolism, growth rate, and age."

The team determined that the small T. rex were growing as fast as modern-day warm-blooded animals such as mammals and birds. Woodward and her colleagues also found that by counting the annual rings within the bone, much like counting tree rings, Jane and Petey were teenaged T.rex when they died; 13 and 15 years old, respectively.

There had been speculation that the two small skeletons weren't T. rex at all, but a smaller pygmy relative Nanotyrannus. Study of the bones using histology led the researchers to the conclusion that the skeletons were juvenile T. rex and not a new pygmy species.

Instead, Woodward points out, because it took T. rex up to twenty years to reach adult size, the tyrant king probably underwent drastic changes as it matured. Juveniles such as Jane and Petey were fast, fleet footed, and had knife-like teeth for cutting, whereas adults were lumbering bone crushers. Not only that, but Woodward's team discovered that growing T. rex could do a neat trick: if its food source was scarce during a particular year, it just didn't grow as much. And if food was plentiful, it grew a lot.

"The spacing between annual growth rings record how much an individual grows from one year to the next. The spacing between the rings within Jane, Petey, and even older individuals is inconsistent -- some years the spacing is close together, and other years it's spread apart," said Woodward.

The research by Woodward and her team writes a new chapter in the early years of the world's most famous dinosaur, providing evidence that it assumed the crown of tyrant king long before it reached adult size.